Application

Anti-Sir2 (AS-16) antibody produced in rabbit has been used in western blotting immunoprecipitationantibody microarray

Biochem/physiol Actions

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). Sir2 protein is an NAD-dependent histone deacetylase. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetyl ADP-ribose. Through histone deacetylation, Sir2 may silence chromatin. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction.

Disclaimer

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General description

Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.

Immunogen

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.